Serotyping Scheme for Campylobacter jejuni and Campylobacter coli Based on Direct Agglutination of Heat-Stable Antigens
نویسندگان
چکیده
منابع مشابه
Serotyping scheme for Campylobacter jejuni and Campylobacter coli based on direct agglutination of heat-stable antigens.
Campylobacter is now the most frequently reported cause of gastrointestinal disease in England and Wales, yet few isolates are characterized beyond the genus level. The majority of isolates are Campylobacter jejuni (90%), with most of the remainder being Campylobacter coli. We describe an adaptation of the Penner serotyping scheme in which passive hemagglutination has been replaced by detection...
متن کاملEvaluation of commercial antisera for serotyping heat-labile antigens of Campylobacter jejuni and Campylobacter coli.
Commercial antisera for serotyping 22 heat-labile antigens of Campylobacter jejuni and Campylobacter coli were evaluated by using 66 isolates from human and nonhuman sources. Test results were compared with results of tests using antisera produced at the Centers for Disease Control (CDC), Atlanta, Ga. All strains (three isolates of each of the 22 serotypes) were typeable with the CDC antisera. ...
متن کاملTyping of heat-stable and heat-labile antigens of Campylobacter jejuni and Campylobacter coli by coagglutination.
A coagglutination system has been devised for typing heat-stable and heat-labile antigens of Campylobacter jejuni and C. coli. The use of protein A-positive Staphylococcus aureus cells carrying Campylobacter sp. serotype antibody and the treatment of Campylobacter sp. cells with DNase in the antigen suspension permitted rapid and specific coagglutination of rough (autoagglutinable) as well as s...
متن کاملSerotyping of Campylobacter jejuni/coli.
Antisera were prepared from strains of Campylobacter jejuni/coli isolated from patients in six outbreaks of enteritis. Bactericidal antibodies, and agglutinating antibodies to heat-labile and heat-stable antigens, were demonstrated. These reactions were used to type a number of strains isolated from patients in each outbreak, and to distinguish 'epidemic' from 'non-epidemic' strains.
متن کاملDetection of heat-stable antigens of Campylobacter jejuni and C. coli by direct agglutination and passive hemagglutination.
The two serotyping schemes for the detection of heat-stable antigens of Campylobacter jejuni and Campylobacter coli use the same strains for antiserum production but differ in the detection systems used for identifying agglutination. The Penner method uses passive hemagglutination (PHA) while the Laboratory of Enteric Pathogens method uses the same antisera but in a whole-bacterial-cell direct ...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 1998
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.36.2.335-339.1998